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What is an enzymatic FRET biosensor?
Posted May 15, 2024
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes
Answer

An enzymatic FRET biosensor is a molecular tool designed to detect and measure the activity of enzymes using FRET. It typically consists of a donor fluorophore, an acceptor fluorophore, and a recognition element that interacts with the enzyme of interest. 

Additional resources
Application of FRET Biosensors in Mechanobiology and Mechanopharmacological ScreeningFörster Resonance Energy Transfer (FRET) Screen Quest™ TR-FRET No Wash cAMP Assay Kit FMOC-Asp(5-FAM)-OH

What organic dyes are commonly used as fluorescent probes in FRET?What factors affect the degree of spectral overlap?What techniques can be used for FRET quantification?What methods are used to measure FRET for cell biology studies?What should I consider when selecting donor/acceptor FRET pairs?