What are some common sources of error in cell viability assays?

There are various sources of error in cell viability assays. Experimental conditions such as temperature, humidity, and pH can all affect cell viability assay and cause inaccurate results. Thus, it is important to have consistent and proper experimental conditions throughout the assay. Another source of error involves cell culture handling. Overgrowth, undergrowth, or contamination of cell culture may all alter viability assay results. Various compounds or chemicals may interact with the assay reagents, affecting the measured viability reading. Improper handling of samples can lead to errors. For example, excessive exposure to light and excessive storage may affect the cell viability. So it is important to ensure proper storage of these assays.