AAT Bioquest

What are the pros of membrane permeability assays?

Posted June 21, 2023


Pros of membrane permeability assays

  • Membrane permeability assays are generally quick and straightforward to perform.
  • Results can be obtained in a relatively short period of time.
  • Exclusion dye propidium iodide has a very bright color making it easy to distinguish live cells from dead cells.
  • Membrane permeability assays are versatile in that they can be used with a wide range of cell types. They can also be adapted to many different experimental conditions.
  • The quantitative measure of cell viability and cytotoxicity can be easily obtained by quantifying the extent of membrane permeability. 
  • Many membrane permeability assays utilize commonly available reagents, making them cost-effective options for determining viability and cytotoxicity.
Additional resources

Parallel artificial membrane permeability assay (PAMPA) Is it better than Caco-2 for human passive permeability prediction?

Cell Viability Assays

Trypan Blue, sodium salt *10 mM aqueous solution*