Cross-linking is an enzyme immobilization technique in which enzymes are cross-linked with each other without any support material, so the enzymes act as their carrier. 

Advantages of Cross Linking 

• Produces controllable particle sizes
• Results in a highly stable enzyme with higher resistance to elevated temperatures and organic solvents
• Minimum or no loss of enzymes
• Highly desirable for the purpose of industrial biotransformations because of its high stability, volumetric efficiency, and recycling with optimum catalytic activity