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What are the advantages of direct detection methods in immunohistochemistry (IHC)?
Posted May 2, 2024
Answer
- The process eliminates the need for a secondary reagent and an additional incubation step.
- It is a faster process overall because of the fewer steps involved.
- The primary antibody can be labeled and conjugated directly to an appropriate fluorochrome or enzyme such as alkaline phosphatase or horseradish peroxidase.
- There is a smaller chance of a non-specific signal.
- Designing multicolor experiments is easy because a wide variety of fluorochromes is easily available.
- Is the recommended technique for detecting highly expressed antigens.