AAT Bioquest

What are the advantages of end-point reverse transcription polymerase chain reaction (RT-PCR)?

Posted November 8, 2023


End-point RT-PCR analysis, which is based on the plateau phase of PCR reaction, is used to analyze amplified products after all the cycles of the PCR reaction have been completed. In this method, amplicons are separated by agarose gel electrophoresis and visualized using a DNA binding dye, such as ethidium bromide (EtBr), to determine the size of the DNA molecules in the range of 500 to 30,000 bp. The advantages of end-point RT-PCR are:

  • Convenient: suitable for the analysis of any double -strand DNA sequence
  • Economical: cheaper than using custom-designed probes.
Additional resources

Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA

Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*

ReadiUse™ dNTP Mix *10 mM*