There are several advantages of fed-batch over batch culture. One advantage is that by gradually feeding nutrients into the bioreactor, the cells can continue synthesizing products over a prolonged period. Another advantage is that the ability to carefully control nutrient availability enables us to promote desired metabolic pathways within the cells, leading to increased product yields and improved productivity. A third advantage is that fed-batch operation allows for the utilization of the bioreactor even during periods when it would typically be inactive or undergoing preparation for the next batch. Additionally, boosting the population of cells leads to a higher biomass concentration, which directly correlates with increased product efficiency.