Image cytometry techniques combine the advantages of microscopic imaging methods and computer aided image analysis. These techniques allow researchers to preferentially analyze adherent cells. They are used extensively in a variety of fields including genomic profiling that require high-content screening and high throughput and in drug discovery.
Advantages of image cytometry techniques:
Can accommodate most staining techniques developed for flow cytometry
Allows researchers to easily quantify the spatial features of individual cells such as molecular localizations and morphological characteristics
Lowers the risk of protein expression changes due to dissociation
Cells are not subjected to undue physical stress during analysis
Cells can continue to be cultured after the analysis as cells and cellular features can be identified with minimal or zero staining by taking advantage of artificial intelligence
Protein localization and cell morphology data are maintained and can be integrated into quantitative analyses
The same cells can be monitored and tracked over a period of time, which is valuable for various applications including cell differentiation and drug discovery