Image cytometry techniques combine the advantages of microscopic imaging methods and computer aided image analysis. These techniques allow researchers to preferentially analyze adherent cells. They are used extensively in a variety of fields including genomic profiling that require high-content screening and high throughput and in drug discovery.
Advantages of image cytometry techniques:
- Can accommodate most staining techniques developed for flow cytometry
- Allows researchers to easily quantify the spatial features of individual cells such as molecular localizations and morphological characteristics
- Lowers the risk of protein expression changes due to dissociation
- Cells are not subjected to undue physical stress during analysis
- Cells can continue to be cultured after the analysis as cells and cellular features can be identified with minimal or zero staining by taking advantage of artificial intelligence
- Protein localization and cell morphology data are maintained and can be integrated into quantitative analyses
- The same cells can be monitored and tracked over a period of time, which is valuable for various applications including cell differentiation and drug discovery