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AAT Bioquest

What are the advantages of multiplex PCR?

Posted February 28, 2024


Answer

There are several advantages of multiplex PCR. Firstly, it allows researchers to obtain more information from a single sample by simultaneously amplifying multiple target sequences in a single reaction. This not only conserves samples but also reduces the time and resources required for analysis. Cost-effectiveness is another advantage of multiplex PCR. By amplifying multiple targets in a single reaction, it reduces the consumption of reagents such as dNTPs and enzymes. Multiplex PCR is also time effective, and requires less input material, making it suitable for applications where sample availability is limited. Furthermore, multiplex PCR offers increased accuracy of data analysis by allowing for the simultaneous detection of multiple targets within the same sample. This minimizes the potential for errors associated with separate reactions and simplifies the interpretation of results. Lastly, by carrying out multiple reactions into a closed environment, multiplex PCR reduces the risk of contamination.  

Additional resources

Multiplex Polymerase Chain Reaction

Polymerase Chain Reaction (PCR)

Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*