Multiplexing techniques in real time PCR (qPCR) involve amplifying two or more target genes in the same reaction using the same reagent mix.

Advantages of multiplexing techniques in qPCR include: 

• More information with smaller amount of sample
• More cost effective because it requires fewer enzymes, dNTPs, and other consumables
• Less time required to set up experiments and analyze results  
• Higher throughput
• Higher accuracy and fewer pipetting errors because of amplifying multiple genes in the same wells