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What are the advantages of tagging fluorescent proteins (FPs) to label target proteins?
Posted December 7, 2023

Answer

There are several advantages of tagging fluorescent proteins to label target proteins. One advantage is that they are able to effectively purify proteins by using affinity chromatography. Another advantage is that they are able to express cells and tissues in situ and provide information in real-time. These tags also allow for specific detection of protein tags, making them highly selective. An additional advantage is that they assist in improving the stability and solubility of the protein of interest. Maltose-binding protein is one example of a tag which increases solubility. Lastly, tags contribute to increased protein yield by reducing degradation and enhancing expression.