What are the differences between GFP and EGFP?

Question

Accepted Answer

Basis of differentiation

GFP

EGFP

Definition

GFP is a wild-type green fluorescent protein that is used in molecular cloning of non-mammalian cells

EGFP is an enhanced form of GFP that can be used on mammalian cells during molecular cloning

Origin

Wild-type, isolated from jellyfish (Aequorea victoria)

Synthesized in the laboratory by creating enhanced mutants

Sensitivity

Low

High

Emission of fluorescence signals

Low

High

Uses

Used in mammalian systems

Excitation peak

Major peak at 395 nm, minor peak at 475 nm

Single excitation peak at 490 nm

Fluorescence at 37°C

Fluoresces less intensely than EFGP at 37°C

Fluoresces intensely and matures quickly at 37°C  compared to wild-type GFP

Folding efficiency  at 37°C

Folding efficiency is low at 37°C

Folding efficiency is high at 37°C

Basis of differentiation	GFP	EGFP
Definition	GFP is a wild-type green fluorescent protein that is used in molecular cloning of non-mammalian cells	EGFP is an enhanced form of GFP that can be used on mammalian cells during molecular cloning
Origin	Wild-type, isolated from jellyfish (Aequorea victoria)	Synthesized in the laboratory by creating enhanced mutants
Sensitivity	Low	High
Emission of fluorescence signals	Low	High
Uses	Used in mammalian systems	Used in mammalian systems
Excitation peak	Major peak at 395 nm, minor peak at 475 nm	Single excitation peak at 490 nm
Fluorescence at 37°C	Fluoresces less intensely than EFGP at 37°C	Fluoresces intensely and matures quickly at 37°C compared to wild-type GFP
Folding efficiency at 37°C	Folding efficiency is low at 37°C	Folding efficiency is high at 37°C