What are the differences between GFP and YFP?

Question

Accepted Answer

Basis of differentiation

GFP

YFP

Definition

GFP is a wild-type green fluorescent protein that is used in molecular cloning of non-mammalian cells

YFP (Yellow fluorescent protein) is a genetic mutant of GFP

Color of emitted light

Emits green color light

Emits yellow color light

Excitation peak

Major peak is at 395nm, while the minor peak is at 475 nm

Excitation peak is at 514 nm

Emission peak

Emission peak is at 509 nm

Emission peak is at 527 nm

Significance

Is used as a reporter of expression and to visualize the localization of fused proteins

Used as non-invasive intracellular pH biosensors or fluorescence indicators for Ca2+ concentrations

Basis of differentiation	GFP	YFP
Definition	GFP is a wild-type green fluorescent protein that is used in molecular cloning of non-mammalian cells	YFP (Yellow fluorescent protein) is a genetic mutant of GFP
Color of emitted light	Emits green color light	Emits yellow color light
Excitation peak	Major peak is at 395nm, while the minor peak is at 475 nm	Excitation peak is at 514 nm
Emission peak	Emission peak is at 509 nm	Emission peak is at 527 nm
Significance	Is used as a reporter of expression and to visualize the localization of fused proteins	Used as non-invasive intracellular pH biosensors or fluorescence indicators for Ca2+ concentrations