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AAT Bioquest

What are the differences between GFP and YFP?

Posted November 14, 2022


Answer

Basis of differentiation

GFP

YFP

Definition 

GFP is a wild-type green fluorescent protein that is used in molecular cloning of non-mammalian cells 

YFP (Yellow fluorescent protein) is a genetic mutant of GFP

Color of emitted light

Emits green color light

Emits yellow color light 

Excitation peak

Major peak is at 395nm, while the minor peak is at 475 nm

Excitation peak is at 514 nm 

Emission peak 

Emission peak is at 509 nm 

Emission peak is at 527 nm 

Significance  

Is used as a reporter of expression and to visualize the localization of fused proteins

Used as non-invasive intracellular pH biosensors or fluorescence indicators for Ca2+ concentrations  

Additional resources

A simple and effective method for differentiating GFP and YFP by flow cytometry using the violet laser

Spectrum [YFP]

Extinction Coefficient [YFP]

Purified Rabbit Anti-tagged fusion proteins in all species GFP Antibody *PAb (476), monoclonal*

Purified Rabbit Anti-GFP Antibody *Polyclonal*