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What are the applications for each type of centrifugation technique?
Posted May 9, 2024
Answer
Analytical centrifugation is used to:
- Determine purity of macromolecules in solution
- Track changes in molecular mass of supramolecular complexes
- Establish relative molecular mass of solutes in their native configuration
Differential centrifugation is used to:
- Separate cell membranes and cellular organelles
- Low –resolution nucleus isolation
- Purify extracts containing larger-sized impurities
Density gradient centrifugation is used to:
- Purify large volumes of biomolecules
- Purify viruses for further analysis
- Determine densities of different particles
Rate-zonal density gradient centrifugation is used to:
- Isolate viruses based on the unique size and density of their components
- Isolate, purify, and fractionate DNA molecules from bacteria and viruses
- Fractionate RNA on sucrose gradients
Equilibrium Density Gradient Centrifugation is used to:
- Purify large volumes of biomolecules
- Determine density of different particles
Isopycnic centrifugation is used to:
- Purify large volumes of biomolecules
- Determine densities of different particles
Sucrose Gradient Centrifugation is used to:
- Separate macromolecules such as DNA and RNA
- Determine the size and density of macromolecules
- Analyze protein complexes
Differential Velocity (Moving Boundary) Centrifugation is used to:
- Separate cell membranes and organelles
- Low-resolution nucleus isolation
- Identify and compare particles of varying sizes