What are the applications of reduced representation bisulfite sequencing (RRBS)?

RRBS aids in the identification of individual SNPs and InDel markers, construction of high-density genetic maps and integration of population SNPs markers. It also helps to calculate genetic linkage distance and association analysis for particular traits, making it useful for producing molecular markers. RRBS also is involved in distinguishing linkage groups, screening biased segregation sites, and carrying out QTL mapping based on phenotypic data. It also facilitates sequencing and whole-genome association analysis in specific species populations, aiding in the identification of genome-related regulatory sites for particular traits. Two more applications are that it assists in gene annotation and the creation of detailed genome maps. It also enables population genetic analysis, including assessments of population evolution and gene flow. In clinical settings, it facilitates biomarker discovery by allowing analysis of DNA methylation differences in genomic regions between distinct sample groups, such as diseased and normal tissues.