How do I remove the adaptors from a DNA fragment if there are too many? | AAT Bioquest

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How do I remove the adaptors from a DNA fragment if there are too many?

Posted April 18, 2024

DNA and RNA Quantitation DNA Fragmentation Gene Expression Analysis & Genotyping Nucleic Acids Nucleus Sanger Sequencing

Answer

Purification steps are essential to remove them. Another approach involves magnetic bead-based clean-up, while another option is to purify the products using an agarose gel.

Additional resources

Adapter dimers causes, effects, and how to remove them DNA Fragmentation Gelite™ Green Nucleic Acid Gel Staining Kit

What happens if there are too many adaptors attached to a DNA fragment?What are the differences between short-read sequencing and long-read sequencing?What is long-read sequencing? What are Next Generation Sequencing(NGS) libraries?What is reduced Representation Sequencing (RRS)?