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AAT Bioquest

What are Next Generation Sequencing(NGS) libraries?

Posted April 18, 2024


Answer

An NGS library comprises a collection of DNA fragments of uniform size, each tagged with known adapter sequences at both the 5' and 3' ends. A library represents one sample, and several libraries, each with distinct adapter sequences, can be combined and sequenced together in a single sequencing experiment. 

Additional resources

Library construction for next-generation sequencing: Overviews and challenges

Next Generation Sequencing (NGS)

MagaDye™ 535-ddGTP