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What are Next Generation Sequencing(NGS) libraries?
Posted April 18, 2024
DNA and RNA QuantitationGene Expression Analysis & GenotypingNucleic AcidsNucleusSanger Sequencing
Answer

An NGS library comprises a collection of DNA fragments of uniform size, each tagged with known adapter sequences at both the 5' and 3' ends. A library represents one sample, and several libraries, each with distinct adapter sequences, can be combined and sequenced together in a single sequencing experiment. 

Additional resources
Library construction for next-generation sequencing: Overviews and challengesNext Generation Sequencing (NGS) MagaDye™ 535-ddGTP

Why is sample preparation important for NGS?What are the applications of RNA sequencing?What are the applications of Reduced-Representation Sequencing (RRS)?What happens if there are too many adaptors attached to a DNA fragment?What are the disadvantages of RAD?