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AAT Bioquest

What are the advantages of ddRAD?

Posted April 18, 2024


Answer

ddRAD uses two restriction enzymes for genomic DNA digestion to obtain uniform fragments across the genome. Fragment size selection is performed using electrophoresis. One advantage of this is it enables cost-effective generation of genome-scale data across a wide range of non-model species, facilitating the creation of extensive datasets. 

Additional resources

Optimized double-digest genotyping by sequencing (ddGBS) method with high-density SNP markers and high genotyping accuracy for chickens

Sanger Sequencing

MagaDye™ 535-ddGTP