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What are the differences between AFLP and RFLP?
Posted June 25, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationDNA FragmentationGene Expression Analysis & GenotypingNucleic AcidsNucleus
Answer
Basis of differentiation
AFLP
RFLP
Definition
Is a PCR-based method used to create distinctive genomic fingerprints by selectively amplifying specific DNA fragments after digestion. 
Is a genetic marker that relies on the generation of DNA fragments of varying lengths after enzymes s are treated with specific restriction enzymes 
Cost
Costs more
Costs less
Reliability
Is more reliable
Is less reliable
Sequence knowledge
Sequence knowledge is not required beforehand
Prior sequence knowledge is required
Polymorphism detection efficiency
Has a higher efficiency in detecting polymorphisms
Has a lower efficiency in detecting polymorphisms
Additional resources
The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysisDNA Fragmentation Gel Electrophoresis

What is restriction fragment length polymorphism (RFLP)?What are the similarities between Random Amplified Polymorphic DNA (RAPD) and RFLP?What are the steps involved in Restriction Fragment Length Polymorphism (RFLP)?What is the principle of Restriction Fragment Length Polymorphism (RFLP)?What are AFLP (Amplified Fragment Length Polymorphism)?