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What are the differences between AFLP and RFLP?
Posted June 25, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationDNA FragmentationGene Expression Analysis & GenotypingNucleic AcidsNucleus
Answer

Basis of differentiation

AFLP

RFLP

Definition

Is a PCR-based method used to create distinctive genomic fingerprints by selectively amplifying specific DNA fragments after digestion. 

Is a genetic marker that relies on the generation of DNA fragments of varying lengths after enzymes s are treated with specific restriction enzymes 

Cost

Costs more

Costs less

Reliability

Is more reliable

Is less reliable

Sequence knowledge

Sequence knowledge is not required beforehand

Prior sequence knowledge is required

Polymorphism detection efficiency

Has a higher efficiency in detecting polymorphisms

Has a lower efficiency in detecting polymorphisms

Additional resources
The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysisDNA Fragmentation Gel Electrophoresis

What is restriction fragment length polymorphism (RFLP)?What are the similarities between Random Amplified Polymorphic DNA (RAPD) and RFLP?What are the steps involved in Restriction Fragment Length Polymorphism (RFLP)?What is the principle of Restriction Fragment Length Polymorphism (RFLP)?What are AFLP (Amplified Fragment Length Polymorphism)?