Basis of differentiation | Batch-fed culture | Fed-batch culture |
Definition | In batch culture, a fixed volume of nutrient-rich growth medium is inoculated with a specific microbial culture, and then incubated under controlled conditions | Fed-batch culture refers to a method in biotechnology where nutrients are continuously added to a bioreactor during the cultivation process |
Cultivation system | Closed system | Semi-closed type |
Addition of fresh nutrients | No | Yes |
Volume of culture | Constant | Increases |
Product yield | Low | Medium |
Log phase | Shorter | Longer |
Chance of contamination | Minimal | Medium |
Purpose | Is primarily used for the production of secondary products like antibiotics | It is primarily used for the production of alcohol and recombinant proteins |
Turnover | Because the nutrients and other conditions necessary for microbial growth not replenished, the turnover rate is low | Because the optimal levels of nutrients are being maintained, the turnover rate is medium |