What are the differences between batch and Fed-batch culture?

Basis of differentiation

Batch-fed culture

Fed-batch culture

Definition

In batch culture, a fixed volume of nutrient-rich growth medium is inoculated with a specific microbial culture, and then incubated under controlled conditions

Fed-batch culture refers to a method in biotechnology where nutrients are continuously added to a bioreactor during the cultivation process

Cultivation system

Closed system

Semi-closed type

Addition of fresh nutrients

No

Yes

Volume of culture

Constant

Increases

Product yield

Low

Medium

Log phase

Shorter

Longer

Chance of contamination

Minimal

Medium

Purpose

Is primarily used for the production of secondary products like antibiotics

It is primarily used for the production of alcohol and recombinant proteins

Turnover

Because the nutrients and other conditions necessary for microbial growth not replenished, the turnover rate is low

Because the optimal levels of nutrients are being maintained, the turnover rate is medium