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What are the differences between Shine Dalgarno and Kozak Sequence?
Posted February 2, 2023
BioconjugationCellular Structures and OrganellesDNA and RNA Quantitation
Answer

Shine Dalgarno 

Shine Dalgarno is a ribosomal binding site found commonly in bacterial mRNA and rarely in archaeal mRNA. It is located 8 bases upstream of the start codon AUG. The Shine Dalgarno sequence is 5’AGGAGGU3’. It binds to the smaller 30-S subunit of 70S ribosomes. 

Shine Dalgarno was discovered by Australian scientists John Shine and Lynn Dalgarno. 

Kozak Sequence

Kozak Sequence is a protein translation initiation site found only in eukaryotic mRNA where it surrounds the start codon AUG so that the start codon is present in the Kozak sequence. The Kozak sequence is 5’(gcc)gccRccAUGG3’. It binds to the smaller 40-S subunit of 80S ribosomes. 

Kozak Sequence was discovered by American scientist Marilyn Kosak

Additional resources
Predicting Shine–Dalgarno Sequence Locations Exposes Genome Annotation Errors Cell Structures and Organelles iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate

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