The different types of antibodies available for use in ChIP assays include: polyclonal antibodies, monoclonal antibodies, and epitope-tag antibodies.
Monoclonal antibodiesare generated by a single clone of B cells and recognize a specific epitope with high specificity.
Polyclonal antibodies are produced by multiple clones of B cells and recognize multiple epitopes on the target protein.
Epitope-tag antibodiesare designed to recognize specific tags attached to the target protein. During immunoprecipitation, the protein of interest is typically expressed as a fusion protein with a tag inserted into either the N- or C-terminus.
The most suitable option for a ChIP or ChIP-seq experiment is an antibody that has been validated specifically for ChIP or ChIP-seq applications.