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What are the different types of light used in a flow cytometry experiment?
Posted September 1, 2022
Cell Cycle AssaysCell Viability AssaysCellular ProcessesFlow Cytometry Reagents
Answer
Flow cytometry experiments use different types of lasers as their light source. The most common lasers used in flow cytometers include: 
  • 355 nm Ultraviolet (UV) 
  • 405 – 407 nm Violet 
  • 488 nm Blue
  • 532 nm Green
  • 561 - 568 nm Green-Yellow
  • 633- 640 nm – Red
Additional resources
Flow Cytometry ReagentsLive or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*Flow-cytometric enrichment of Pacific bluefin tuna type A spermatogonia based on light-scattering properties

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