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AAT Bioquest

What is flow cytometry used for?

Posted September 1, 2022


Answer

Flow cytometry is a laboratory, laser-based technique used for identifying and analyzing the chemical and physical characteristics of cells or particles. It involves suspending a sample of cells or particles in fluid, which is then injected into an instrument known as a flow cytometer. A flow cytometer is capable of processing and analyzing approximately 10,000 cells in less than one minute. Flow cytometry is widely used in research for several purposes, including but not limited to: 

●       Counting and sorting cells 

●       Determining cell characteristics and function

●       Detecting biomarkers or characteristics that indicate normal function

●       Observing different leukocyte types

●       Diagnosing, evaluating, and determining potential treatment of bone marrow and blood cancers

●       Evaluating other fluids in the human body

●       Identifying microorganisms such as fungus, bacteria, or yeast

●       Sorting T cells to determine the manner in which an infection may have impacted their function 

Additional resources

Flow Cytometry Reagents

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*