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What are the disadvantages of agarose gel?
Posted April 24, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)
Answer

Disadvantages of agarose gel:

  • Pore size is not uniform
  • Low percentage gels are fragile and likely to break while lifting
  • High percentage gels are generally brittle and likely to set unevenly
  • Can melt during electrophoresis
Additional resources
SDS agarose gels for analysis of proteins Gel Electrophoresis Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

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