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AAT Bioquest

What are the advantages of polyacrylamide gel?

Posted April 24, 2024


Answer

Advantages of polyacrylamide gel:

  • Size of pores is uniform
  • Pore size can be manipulated to increase or decrease molecular sieving
  • Very high resolving power
  • Its chemically synthetic nature results in higher consistency between batches, which gives more reproducible results
  • Is non-reactive with samples because of the absence of charge
  • Resolution time and run time can be manipulated by using different buffers
  • Thin gels can be cast, which leads to more efficient heat dissipation and better separations at higher field strengths
Additional resources

Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting

Gel Electrophoresis

Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*