Why is it important to mix samples/ladders with loading dyes when setting up gel electrophoresis?
Posted April 24, 2024
Answer
Loading dyes are colored dyes that are mixed with a DNA sample before running electrophoresis. They serve three important functions when setting up gel electrophoresis:
- Loading dyes color the sample, simplifying the loading process by providing a color indicator that makes it easier to visualize the sample.
- They act as color indicators during gel electrophoresis, enabling researchers to estimate the distance that the nucleic acid fragments have migrated.
- Loading dyes increase the density of the sample, ensuring that the DNA sinks into the gel and does not diffuse away into the buffer.
Additional resources
Analysis of DNA by Agarose Gel Electrophoresis
Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*