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Why is TAE the preferred choice for gel electrophoresis?
Posted April 24, 2024
Cellular Structures and OrganellesDNA and RNA QuantitationGel ElectrophoresisNucleic AcidsNucleusPolymerase Chain Reaction (PCR)
Answer
Between the TAE and TBE, TAE is preferred for cloning procedures because TBE contains borate, which can inhibit certain enzymes such as ligase. TAE is also preferred for DNA extraction from agarose gel.
Additional resources
Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis Gel Electrophoresis Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

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