AAT Bioquest

What are the disadvantages of cDNA library?

Posted March 1, 2023


A disadvantage of a cDNA library is that it contains only sequences that are present in mature mRNA. Another disadvantage is that introns and other sequences that are altered during transcription are absent in a cDNA library. Sequences such as promoters and enhancers, which are not transcribed into RNA, are not included in the library.

Additional resources

cDNA library construction of two human Demodexspecies

DNA and RNA Quantitation

ReadiScript™ RT Reverse Transcription Kit