A Gram-negative cell wall has the following features:
The outer membrane is separated from the inner cytoplasmic layer by a periplasmic space called the periplasm, which has a network of peptidoglycan chains.
The Gram-negative bacteria has a thin peptidoglycan layer, which is present in the periplasmic space – the area between the inner and outer membranes.
The cell wall is enclosed by a cell envelope, which is composed of 3 layers, including a distinctive outer membrane, a thin peptidoglycan layer, and a cytoplasmic membrane.
The outer membrane of the cell wall is a bilayer structure composed of lipopolysaccharides, phospholipid molecules, lipoproteins, and surface proteins. It protects the cell against harmful microbial agents and damaging environmental elements.
The surface proteins, known as porins, form channels that allow nutrients and other tiny molecules to flow into the cell.
The cell wall stains pink when treated with Gram Stain.