What are the limitations of cell-free protein expression system?
Posted August 15, 2023
One drawback is that these systems post-translational modifications for functional proteins are mostly restricted in E coli cell-free systems. This is because only limited modifications are possible. Another limitation is the lack of a natural membrane interferes with the synthesis of membrane proteins. A third limitation is that cell free systems have a shorter time frame for protein expression compared to in vivo systems. This may result in reduced protein stability.