What are the limitations of live cell imaging? | AAT Bioquest

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What are the limitations of live cell imaging?

Posted April 10, 2024

Cellular Processes Cellular Structures and Organelles Flow Cytometry Reagents Physiological Probes live cell analysis

Answer

Limitations of live cell imaging:

The narrow focal depths of conventional microscopy largely restrict live cell imaging to studying cells on a single plane.
Processes such as photobleaching and phototoxicity, which are essential for live-cell imaging, may interfere with survival and distort the observations.

Additional resources

Live-cell imaging Flow Cytometry Reagents Cell Explorer™ Fixable Live Cell Tracking Kit *Green Fluorescence*

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