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AAT Bioquest

What are the major enzymes used in DNA transcription?

Posted February 23, 2023


Answer

DNA transcription uses three polymerase enzymes – RNA polymerase I, RNA polymerase II, and RNA polymerase III. Although the structures of all three polymerase enzymes are homologous, they perform different functions. 

RNA polymerase I plays a role in the transcription of ribosomal RNA. It is located in the nucleolus. 

RNA polymerase II is located in the nucleus and plays a role in the transcription of mRNA. 

RNA polymerase III is also located in the nucleus and is responsible for the transcription of both ribosomal RNA and transfer RNA. 

Additional resources

DNA wrapping in transcription initiation by RNA polymerase II

DNA and RNA Quantitation

StrandBrite™ Green RNA Quantifying Reagent *200X DMSO Solution*