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AAT Bioquest

What are the pathways of mRNA degradation?

Posted August 3, 2023


Answer

There are two general pathways of mRNA degradation in eukaryotes. In both pathways, the first step involves deadenylation or the exonucleolytic removal of the poly (A) tail. In the first pathway, deadenylation is followed by the hydrolysis of the cap, after which the mRNA body is progressive degraded by a 5' exonuclease. In the second pathway, a complex of 3' exonucleases degrades the mRNA body. This is followed by the hydrolysis of the remaining cap structure.

Additional resources

Messenger RNA Degradation in Bacterial Cells

StrandBrite™ Green RNA Quantifying Reagent *200X DMSO Solution*

Portelite™ Fluorimetric RNA Quantitation Kit*Optimized for Cytocite™ and Qubit™ Fluorometers*

DNA and RNA Quantitation