AAT Bioquest

What are the selection criteria for LAMP primers?

Posted June 17, 2022


Choosing the correct LAMP primers is crucial for obtaining accurate results. LAMP primers should have these properties: 

●       Length of approximately 18 to 24 bases

●       Start and end with 1-2 G/C pairs

●       40% to 60% G/C content

●       Melting temperature (Tm) between 65°C and 75°C

●       The melting temperature difference between primer pairs should not be more than 5°C  

●       Primer pairs should not have complementary regions

Additional resources

Accelerated reaction by loop-mediated isothermal amplification using loop primers

LAMP Green™ *50X DMSO Solution*