logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
What are the selection criteria for LAMP primers?
Posted June 17, 2022

Answer

Choosing the correct LAMP primers is crucial for obtaining accurate results. LAMP primers should have these properties: 

●       Length of approximately 18 to 24 bases

●       Start and end with 1-2 G/C pairs

●       40% to 60% G/C content

●       Melting temperature (Tm) between 65°C and 75°C

●       The melting temperature difference between primer pairs should not be more than 5°C  

●       Primer pairs should not have complementary regions