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AAT Bioquest

What are the similarities between separating gel and stacking gel?

Posted July 31, 2024


Answer

Stacking gel and separating gel are both made from polyacrylamide. Both use a buffer system to generate an electric current that facilitates the movement of charged molecules through the gel. Both are used in polyacrylamide gel electrophoresis (PAGE).

 

Additional resources

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting analyses via colored stacking gels

Gel Electrophoresis

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