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What concentration should I use protease inhibitors?
Posted March 21, 2024
Antibodies and ProteomicsAntibody and Protein LabelingEnzymesProteases
Answer

Protease inhibitor

Optimal concentration

Aprotinin

1 µg/ml (0.15 µM)

Leupeptin

0.5 µg/ml (1 µM)

Pepstatin

0.7 µg/ml (1 µM)

PMSF

85 µg/ml (0.5 mM)

Na2-EDTA

0.35 mg/ml (0.75 mM)

Pefabloc

0.5 mg/ml (2 mM)

Additional resources
Protease Inhibitors 101: How They Work and How to Use ThemProteasesAmplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

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