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What do I dissolve DAPI in?
Posted November 30, 2022
BioconjugationAntibody and Protein LabelingCell Structures and OrganellesImmunohistochemistry (IHC)Nucleus
Answer
DAPI is insoluble in PBS, even with the addition of heat and sonication. The use of buffers is not recommended to be used with DAPI. Instead, water should be used to prepare a stock solution and also be protected from light.