What is FLIM FRET?

Posted May 15, 2024

Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Physiological Probes

Answer

FLIM FRET allows researchers to map both the spatial and temporal variations in the lifetime of fluorescent molecules within a single living cell, going beyond decay curves.

Additional resources

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications Förster Resonance Energy Transfer (FRET) Screen Quest™ TR-FRET No Wash cAMP Assay Kit FMOC-Asp(5-FAM)-OH

What methods are used to measure FRET for cell biology studies?What should I consider when selecting donor/acceptor FRET pairs?What does spectral overlap mean?What organic dyes are commonly used as fluorescent probes in FRET?What factors can influence FRET efficiency?

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