The incubation time for the BCA assay typically ranges from 30 minutes to 2 hours. The exact duration depends on the specific protocol used and the concentration of the protein being measured. The Standard BCA Protein Assay measures protein concentrations between 10 μg/mL and 2000 μg/mL and requires either a 30-minute incubation at 37°C or a 2-hour incubation at room temperature. In contrast, the Enhanced BCA Protein Assay involves a 30-minute incubation at 60°C and is designed to detect proteins within a range of 5 μg/mL to 250 μg/mL.