Basis of differentiation | ELISA assay | |
Definition | Is an immunological technique utilized to detect and quantify antibodies, glycoproteins proteins, and antigens | Is a lab technique used to measure the total amount of protein in a sample |
Specificity | Highly specific to a particular protein or antigen | Measures total protein concentration and is not specific to individual proteins |
Time | Typically 1 to 4 hours depending on the type of ELISA and protocol | 30 minutes to 2 hours, depending on the protocol (Standard or Enhanced) |
Sample type | Requires specific antibodies to detect specific proteins or antigens in a sample | Used to measure total protein concentration; does not require specific antibodies |
Color change | A shift to blue color signifies the presence of the antigen-antibody complex | A deep purple color develops as a result of the reaction between Cu⁺ and bicinchoninic acid |
Complexity | Is more complex; involves coating plates, blocking, incubation with antibodies | Is simpler; involves mixing reagents, incubating, and measuring absorbance |