What is the principle of multiplex PCR?

The principle of multiplex PCR is to include multiple pairs of primers in the reaction mixture, each specific to a different target sequence. The primers bind to their correlating DNA templates, both in combinations and individual pairs. 

This specific approach allows for efficient and cost-effective analysis of multiple targets, saving time and resources compared to running individual PCR reactions for each target sequence. The multiplex PCR reaction conditions, including annealing temperatures, primer concentrations, and cycle parameters, are optimized to ensure the successful simultaneous amplification of multiple DNA fragments. Moreover, this approach for more possibilities in PCR conditions and primer design, making it a faster option compared to conventional PCR.