What is the significance of running an uncut DNA on electrophoresis gel?
Posted May 2, 2023
- Confirms plasmid purity – An uncut plasmid will run as a single band on the gel, which indicates that no contaminants are present in the plasmid preparation.
- Helps assess plasmid quantity – The plasmid concentration in the preparation can be estimated based on the intensity of the uncut plasmid band. This is especially important for downstream applications such as transformation or transfection.
- Acts as a quality control measure – Running an uncut plasmid on the gel is a vital quality control step that ensures the plasmid preparation is suitable for downstream applications.