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What are the limitations of in-gel staining?
Posted May 2, 2023
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel
Answer

3 main limitations of in-gel staining include: 

  • Dye cannot be used more than once
  • Sample mobility may be altered and samples may not run true to size because of the dye binding to nucleic acids
  • Higher likelihood that the dye may run off after a long run
Additional resources
Staining Proteins in Gels Gel Electrophoresis Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

What are the limitations of post-electrophoresis staining?What are the advantages of in-gel staining?Which technique is used to visualize DNA following a PCR reaction?How does the size of the DNA fragment affect its movement or migration through the agarose gel during electrophoresis?How is a DNA ladder visualized in gel electrophoresis?