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What are the limitations of post-electrophoresis staining?
Posted May 2, 2023
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel
Answer

Main limitations of post-electrophoresis staining include: 

  • Larger amounts of dye required
  • Adds time to the workflow
  • More hazardous waste generated when using dyes such as ethidium bromide
Additional resources
Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review Gel Electrophoresis Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

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