AAT Bioquest

What is the typical concentration for dNTPs?

Posted February 17, 2023


dNTPs comprises 4 basic nucleotides - dATP, dCTP, dGTP, and dTTP that work as building blocks of new DNA strands. The typical concentration for each of the four dNTPs is between 40μM and 200μM. The exact concentration for an experiment will depend on the type of experiment being carried out. Too high concentrations of dNTP can hinder the PCR and prevent the formation of products. It has been found, however, that higher concentrations up to 400 μM of each dNTP work effectively. Higher dNTP concentration may be required when amplifying large PCR fragments. In general, lower concentrations of dNTP are preferable as they ensure lower background and cleaner product. Large changes in dNTP concentrations may require corresponding changes in MgCl2 concentrations. 

Additional resources

Optimisation of the polymerase chain reaction

Polymerase Chain Reaction (PCR)

ReadiUse™ dNTP Mix *10 mM*