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AAT Bioquest

What markers can be used to identify Treg cells?

Posted March 5, 2024


Answer

Most common markers used to identify Treg cells are: 

  • CD3: CD3 is a cell surface protein complex found on T cells and is widely used to identify Treg cells.  
  • CD4: CD4 is usually expressed along with other regulatory markers on Tregs and is used in conjunction with other markers to identify Treg cells.
  • CD25: CD25 is highly expressed on the surface of Treg cells and serves as a powerful marker for their identification.  
  • CD127: CD127 is expressed at low levels on a substantial number of Treg cells and is a key marker of Treg cells in human peripheral blood. 
  • FoxP3: FOXP3 is a specific marker for naturally occurring Tregs or natural Tregs. 

These molecular markers are widely implemented in clinical and research settings to identify and analyze Treg cells using flow cytometry and other immune profiling techniques. 

Additional resources

Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry

Cluster of Differentiation (CD Antibodies)

PE/Texas Red® Anti-human CD4 Antibody *RPA-T4*