AAT Bioquest

What markers can I use to measure oxidative stress?

Posted June 30, 2023


There are several markers one can use to measure oxidative stress. DHE is used to oxidize superoxide radicals to form a fluorescent product, indicating the presence of ROS. Another marker is DCFH-DA, which is converted to DCF in the presence of ROS. Amplex UltraRed reagent reacts with hydrogen peroxide in the presence of horseradish peroxidase, to produce a fluorescent signal. 

Protein carbonyl content is a commonly used marker for oxidative modification of proteins and can be quantified using the DNPH assay. Nitration of tyrosine residues in proteins can be detected by using specific antibodies against 3-tyrosine. Malondialdehyde is the most commonly used lipid marker of oxidative stress. MDA is a byproduct of lipid peroxidation and can be measured using TBARS assay or HPLC.  

Additional resources

Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Reactive Oxygen Species (ROS)

DCFH-DA [2',7'-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*

Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit