There are several markers one can use to measure oxidative stress. DHE is used to oxidize superoxide radicals to form a fluorescent product, indicating the presence of ROS. Another marker is DCFH-DA, which is converted to DCF in the presence of ROS. Amplex UltraRed reagent reacts with hydrogen peroxide in the presence of horseradish peroxidase, to produce a fluorescent signal. 

Protein carbonyl content is a commonly used marker for oxidative modification of proteins and can be quantified using the DNPH assay. Nitration of tyrosine residues in proteins can be detected by using specific antibodies against 3-tyrosine. Malondialdehyde is the most commonly used lipid marker of oxidative stress. MDA is a byproduct of lipid peroxidation and can be measured using TBARS assay or HPLC.