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AAT Bioquest

What's the importance of protease inhibitor cocktails?

Posted March 21, 2024


Answer

Protease inhibitor cocktails are crucial for protein quantification studies as they prevent protease activity, ensuring accurate protein purification and yield. Protease inhibitors vary based on the type of proteases they target (such as serine, cysteine or aspartic proteases). Thus, sometimes a single inhibitor may not be sufficient, so using a designed cocktail of inhibitors can provide better protection for proteins in experiments. Additionally, using pre-made cocktails can be cost-effective compared to assembling individual inhibitors. By purchasing only the necessary amount of inhibitors in a ready-to-use format, researchers can avoid overspending on excess reagents that may go unused. Lastly, premade protease inhibitor cocktails offer consistent results as they are already tested and optimized for various experimental conditions. They are produced in larger batches, ensuring uniformity across different batches and saving time.

 

Additional resources

Effect of protease inhibitors on the quantitative and qualitative assessment of oral microbes

Proteases

Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*