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What should be considered when I do in-gel staining?
Posted May 2, 2023
Agarose gel electrophoresisDNA and RNAGel ElectrophoresisNucleic AcidsNucleusProteinpolyacrylamide gel
Answer

When doing in-gel staining, it’s advisable to compare the benefits and limitations of the technique. 

Benefits: In gel staining is more convenient, has a faster workflow, and requires less stain for visualization. 

Limitations: Sample mobility may be altered and the samples may not run true to size because of the dye binding to nucleic acids. This can make it difficult to detect samples of lower molecular weight, especially during a long run. Moreover, dyes can only be used once and may run off after a long run. 

Additional resources
Modification in Silver Staining Procedure for Enhanced Protein Staining Gel Electrophoresis Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*

Which technique is used to visualize DNA following a PCR reaction?How do DNA fragments move through the gel?What are the advantages of in-gel staining?What are the limitations of post-electrophoresis staining?How does the size of the DNA fragment affect its movement or migration through the agarose gel during electrophoresis?